Isolation and Characterization of Protoporphyrin IX from Bacterial Catalase
نویسندگان
چکیده
منابع مشابه
Isolation and characterization of protoporphyrin IX from bacterial catalase.
Catalase has been isolated and crystallized from the bacterium, Micrococcus lysodeikticus, but the prosthetic group of the enzyme was identified only by indirect means (I). The method described by Herbert and Pinsent (1) for the isolation of catalase from bacteria employed 78 liters of starting material. This procedure could not be applied with equal success when the starting material consisted...
متن کاملCharacterization and standardization of tissue-simulating protoporphyrin IX optical phantoms.
Optical devices for measuring protoporphryin IX (PpIX) fluorescence in tissue are routinely validated by measurements in optical phantoms. Yet there exists limited data to form a consensus on the recipe for phantoms that both mimic the optical properties found in tissue and yield a reliable and stable relationship between PpIX concentration and the fluorescence remission intensity. This study c...
متن کاملIsolation and characterization of different bacterial agents from crayfish (Astacus leptodactylus) of Aras reservoir, Iran
Freshwater crayfish (Astacus leptodactylus) is an important economic fisheries resource of Aras reservoir, Iran. The infection of freshwater crayfish by gram negative and gram positive bacteria are common in natural and cultural environments. These bacteria are considered as secondary disease agents or opportunities. This study was conducted to investigate infes...
متن کاملSimultaneous liquid-chromatographic determination of zinc protoporphyrin IX, protoporphyrin IX, and coproporphyrin in whole blood.
We describe a method for simultaneously measuring concentrations of coproporphyrin, zinc protoporphyrin IX, and protoporphyrin IX in whole blood by liquid chromatography, with use of reversed-phase ion-pair system, fluorometric detection, and internal standardization. Each analysis requires 10 microL of whole blood and 15 min total analysis time. Analytical recovery ranged from 84 to 92%, day-t...
متن کاملFluorescence tomography characterization for sub-surface imaging with protoporphyrin IX.
Optical imaging of fluorescent objects embedded in a tissue simulating medium was characterized using non-contact based approaches to fluorescence remittance imaging (FRI) and sub-surface fluorescence diffuse optical tomography (FDOT). Using Protoporphyrin IX as a fluorescent agent, experiments were performed on tissue phantoms comprised of typical in-vivo tumor to normal tissue contrast ratios...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)81351-9